Anti-Mouse IFNα (Clone TIF-3C5) – Purified <i>in vivo </i>GOLD™ Functional Grade - 1.0 mg

B, FA, in vivo, N, WB

Interferon Alpha

Next Day 2 - 8°C

IFNα antibody, TIF-3C5, recognizes interferon (IFN)-α, a pleiotropic cytokine belonging to the type I IFN family of cytokines. IFNα is induced following recognition of microbial products via pattern-recognition receptors (PRRs). Hematopoietic cells, particularly plasmacytoid dendritic cells (pDCs), are the predominant source of IFNα following stimulation1, 2. IFNα binds to the ubiquitously expressed common type I IFN receptor (IFNAR) that consists of the α-chain (IFNAR1) and the β-chain (IFNAR2), ultimately resulting in the transcription of various IFN-stimulated genes (ISGs) that contribute to antiviral immunity. ISGs directly promote antipathogenic activity by inhibiting viral entry, transcription, translation, and assembly in host cells. ISGs also synergize with other cytokines to activate innate effector cells, such as NK cells and dendritic cells (DCs), augment host adaptive immune responses, and enhance antigen presentation3. IFNα plays a role in the pathogenesis of autoimmune diseases, such as systemic lupus erythematosus (SLE)4. In addition, IFNα has multiple anti-tumor properties, including direct cytotoxicity of tumor cells and stimulating innate and adaptive immune cells, and IFNα is FDA-approved for the treatment of multiple cancers5.

FA
B
N
WB

In Vitro Activity of TIF-3C5 - Inhibits IFNα-induced (IFNα-A, -1, -4, -5, -11 and 13) Stat1 phosphorylation in vitro, (TIF-1D6 does not block in vitro functional activity). Blocks IFNα (IFNα-A, -1, -4, -5, -11 and 13) induction of MHC-I expression (H2-Kb) using L929 or fibrosarcoma cell lines in a dose dependent manner. Neutralizes IFNα-induced antiviral activity in vitro following infection of L929 cells with VSV. Functional activity of both recombinant (IFNα-A, -1, -4, -5, -11 and 13) and natural IFNα can be blocked with no neutralization of IFNg or IFNb.
In vivo Activity of TIF-3C5- TIF-3C5 circulates with a half-life of 4 days- blockade of IFNa by TIF-3C5 increases the lethality of mice infected with West Nile Virus(WNV), similar to susceptibility of Irf7 -/- mice.

Apoptosis, Cell Biology, Cell Death, Signal Transduction, Tumor Suppressors

AB_2737533

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

IFNα is expressed by hematopoietic cells, predominantly plasmacytoid dendritic cells (pDCs), following stimulation.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.