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Anti-Human IgG Fc Specific (Clone HP6043) – Purified in vivo GOLD™ Functional Grade - 0.25 mg

ArtNr LEIN-I-1193-0.25mg
Hersteller Leinco Technologies
Menge 0.25 mg
Kategorie
Typ Antibody Monoclonal
Applikationen FC, ELISA
Clon HP6043
Specific against Human (Homo sapiens)
Host Mouse
Purity ≥95% monomer by analytical SEC, > 95% by SDS Page
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Applications
Quality Tested by Leinco FC This antibody has been quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume.
ELISA This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml.
Manufacturer - Category
Purified in vivo Functional Grade>Gold Purified Bulk Antibodies|Human Immunoglobulins
Manufacturer - Targets
Fc Fragment Specific, Human Immunoglobulin, IgG
Country of Origin
USA
Shipping Temperature
Next Day 2 - 8°C
Storage Conditions
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.
Cross Reactivity
Cross-reactivity by ELISA against human myeloma proteins:
Human IgG1: 100%
Human IgG2: 100%
Human IgG3: 100%
Human IgG4: 100%
Human IgM: < 0.2%
Human IgA: < 0.1%
Human IgE: < 0.1%
There is no detectable binding to bovine, goat, horse sheep IgG.
NOTE: This Anti-Human IgG, Fc Fragment Specific (Clone HP6043) when conjugated to HRPO is validated for use in Enzyme Immunoassay for the detection of Human IgG, Fc Fragment Specific (Part No.: I-1200)
PubMed
Fc Fragment Specific
Manufacturer - Research Area
Cell Biology, Signal Transduction, Stem Cell
Manufacturer - Specificity
Mouse Anti-Human IgG, Fc Fragment Specific (Clone HP6043) recognizes Human IgG, Fc Fragments. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
RRID
AB_2893813
Concentration
1.0 - 5.0 mg/ml
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Endotoxin Level
≤ 1.0 EU/mg as determined by the LAL method
Additional Information
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords
Monoclonal Antibody, IgG, Fc Fragment Specific, HP6043, Human, Anti-Human IgG Fc Specific - Purified Functional Grade, GOLD

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 0.25 mg
Lieferbar: In stock
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