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Anti-Human IgG Fc Specific (Clone HP6043) – Purified in vivo GOLD™ Functional Grade - 0.25 mg

Anti-Human IgG Fc Specific (Clone HP6043) – Purified in vivo GOLD™ Functional Grade - 0.25 mg

Item no.	LEIN-I-1193-0.25mg
Manufacturer	Leinco Technologies
Amount	0.25 mg
Category	In Vivo Antibodies Flow Cytometry ELISA Primary Antibodies Immunostaining
Type	Antibody Monoclonal
Applications	FC, ELISA
Clone	HP6043
Specific against	Human (Homo sapiens)
Host	Mouse
Purity	≥95% monomer by analytical SEC, > 95% by SDS Page
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping Condition	Cool pack
Available
Manufacturer - Applications	Quality Tested by Leinco FC This antibody has been quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. ELISA This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml.
Manufacturer - Category	Purified in vivo Functional Grade>Gold Purified Bulk Antibodies\|Human Immunoglobulins
Manufacturer - Targets	Fc Fragment Specific, Human Immunoglobulin, IgG
Country of Origin	USA
Shipping Temperature	Next Day 2 - 8°C
Storage Conditions	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.
Cross Reactivity	Cross-reactivity by ELISA against human myeloma proteins: Human IgG1: 100% Human IgG2: 100% Human IgG3: 100% Human IgG4: 100% Human IgM: < 0.2% Human IgA: < 0.1% Human IgE: < 0.1% There is no detectable binding to bovine, goat, horse sheep IgG. NOTE: This Anti-Human IgG, Fc Fragment Specific (Clone HP6043) when conjugated to HRPO is validated for use in Enzyme Immunoassay for the detection of Human IgG, Fc Fragment Specific (Part No.: I-1200)
PubMed	Fc Fragment Specific
Manufacturer - Research Area	Cell Biology, Signal Transduction, Stem Cell
Manufacturer - Specificity	Mouse Anti-Human IgG, Fc Fragment Specific (Clone HP6043) recognizes Human IgG, Fc Fragments. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
RRID	AB_2893813
Concentration	1.0 - 5.0 mg/ml
Formulation	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Endotoxin Level	≤ 1.0 EU/mg as determined by the LAL method
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords	Monoclonal Antibody, IgG, Fc Fragment Specific, HP6043, Human, Anti-Human IgG Fc Specific - Purified Functional Grade, GOLD

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-I-1193-0.25mg-safety-datasheet.pdf