Anti-Human CD195 (CCR5) [Clone HEK/1/85a/7a] – Purified - 50 µg

Quality Tested by Leinco FC The suggested concentration for this CD195 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 10⁶ cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.

CD195

Next Day 2 - 8°C

CCR5 antibody, HEK/1/85a/7a, recognizes C-C chemokine receptor type 5 (CCR5), also known as CD195, CC-CKR-5, CHEMR13, and HIV-1 fusion coreceptor. CCR5 is a 45 kDa seven-transmembrane G-protein-coupled receptor (GPCR). CCR5 is expressed on a subset of memory T cells, macrophages, and immature dendritic cells (DCs) and is upregulated by proinflammatory cytokines1, 2. CCR5 binds to inflammatory CC-chemokines, including CCL3 (macrophage inflammatory protein (MIP)-1α), CCL4 (MIP-1β), and RANTES, which increases intracellular calcium levels and results in cytoskeletal rearrangement and chemotactic cell migration3. CCR5 is also the coreceptor for HIV-1 following binding of viral gp120 to CD44-7. The levels of CCR5 on cells influence the rate of entry of HIV-1, and patients with lower levels of CCR5 exhibit immunity against infection8. CCR5 also contributes to other human diseases, including type I diabetes9 and cancer10.

ICC

Clone HEK/1/85a/7a recognizes an epitope on human CCR5.

0.5 mg/ml

CCR5 is expressed on memory T cells, macrophages, and immature dendritic cells (DCs).

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.