Anti-Mouse IL-21R (Clone 4A9) - Purified in vivo GOLD™ Functional Grade

Purified in vivo Functional Grade>Gold Purified Bulk Antibodies|Monoclonal Antibodies|Purified in vivo Functional Grade|Purified in vivo Functional Grade

2 - 8°C Wet Ice

Interleukin-21 receptor (IL-21R) is a single pass type I membrane protein and a member of the type I cytokine receptor family. It is the receptor for Interleukin-21 (IL-21) and upon binding forms a heterodimer with the common gamma subunit (CD132; IL-2RG). IL-21R and IL-21 are important for the proliferation and differentiation of B cells, T cells, and natural killer cells. They function in survival signalling for antiviral CD8+ T cells during chronic infections and induce growth and differentiation of mature B cells in Ig-secreting plasma cells. Additionally, they prevent T cell exhaustion and viral persistence by limiting virus driven Treg cell proliferation1. IL-21 and IL21-R function as early as the pro-B cell stage, with receptor-mediated signalling increasing as cells mature2. When IL-21R binds IL-21, the JAK/STAT transduction pathway is activated.
4A9 was generated by immunizing Lewis rats with IL-21R-transfected YB2/0 and subsequent fusion of immunized spleen cells to SP2/03. FACS analysis and immunoprecipitation studies show that 4A9 selectively binds to IL-21R. Additionally, 4A9 stains the majority of spleen cells from wild type C57BL/6 mice but not IL-21R deficient mice. IL-21 blocks the binding of 4A9 to IL-21R-transfected YB2/0 cells.

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

Rat YB2/0 hybridoma transfectant cell line expressing truncated IL-21R