Anti-Human IL-2 Rβ - 25 µg

CyTOF®, FC, IHC FFPE, N

IL-2 R beta

Next Day Ambient

CD122 is a 70-75 kD IL-2 receptor β chain that is a type I membrane protein. CD122 is involved in T cell-mediated immune responses and its activation increases proliferation of CD8+ effector T cells. It exists in three forms with varying degrees of binding affinity with IL-2. The low affinity form is a monomer of the α subunit and has no involvement in signal transduction. The intermediate affinity form is a γ/β heterodimer and the high affinity form is an α/β/γ heterotrimer. The intermediate and high affinity forms of the receptor are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. This protein also interacts with the IL-15 receptor.

IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 2 - 15 µg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
Neutralization: This antibody is useful for neutralization of Human IL-2 Rβ bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human IL-2 Rβ (at 30 ng/ml) is 0.05-0.15 µg/ml.
CyTOF-ready:Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Clone 27302 recognizes an epitope on human IL-2 Rβ. This antibody does not cross-react with rhIL-2 Rα, rhγc, rhIL-4 R or rhIL-6 R.

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

< 0.1 EU/µg as determined by the LAL method

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.