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Anti-Human LIF - 25 µg

Anti-Human LIF - 25 µg

ArtNr	LEIN-L156-25ug
Hersteller	Leinco Technologies
Menge	25 ug
Quantity options	100 ug 25 ug
Kategorie	Metabolism Diabetes Glucose Homeostasis Antibodies Obesity Immunohistochemistry Primary Antibodies
Typ	Antibody Primary
Applikationen	IHC
Specific against	Human (Homo sapiens)
Host	Caprine
NCBI	3976
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Versandbedingung	Raumtemperatur
Lieferbar
Manufacturer - Applications	IHC FFPE, N
Manufacturer - Category	Polyclonal Antibodies>Growth Factors & Cytokines
Manufacturer - Targets	LIF
Country of Origin	USA
Shipping Temperature	Next Day Ambient
Storage Conditions	The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Product Description	Leukemia inhibitory factor (LIF) (22 kDa (predicted) glycoprotein) is a pleiotropic cytokine that belongs to the IL-6 receptor family. It binds to a heterodimeric membrane receptor made up of a LIF-specific subunit, gp190, and the subunit gp130. Human LIF acts through a receptor comprising a 190 kDa LIF-binding alpha -chain and a 130 kDa signal-transducing beta -chain (gp130), which is shared with the other members of the IL-6 family.
Background	Leukemia inhibitory factor (LIF) (22 kDa (predicted) glycoprotein) is a pleiotropic cytokine that belongs to the IL-6 receptor family. It binds to a heterodimeric membrane receptor made up of a LIF-specific subunit, gp190, and the subunit gp130. Human LIF acts through a receptor comprising a 190 kDa LIF-binding alpha -chain and a 130 kDa signal-transducing beta -chain (gp130), which is shared with the other members of the IL-6 family.
Other Applications Reported In Literature	IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 5-15 µg/ml. Neutralization:Measured by its ability to neutralize LIF-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically 0.04-0.08 µg/mL in the presence of 3 ng/mL Recombinant Human LIF.
PubMed	LIF
Manufacturer - Specificity	Goat Anti-Human Leukemia Inhibitory Factor recognizes Human LIF by ELISA. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. By ELISAs, less than 50% cross-reactivity with recombinant mouse LIF was observed.
RRID	AB_2831130
Formulation	This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Antigen Distribution	Expressed in the trophectoderm of the developing embryo, activated CD4+ T cells.
Endotoxin Level	< 0.1 EU/µg as determined by the LAL method
Immunogen	E. coli-derived recombinant human LIF (Acession No.: P15018)
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords	Polyclonal Antibody, LIF, Leukemia Inhibitory Factor, CDF, DIA, Human Leukemia Inhibitory Factor, Human LIF, CDF, D factor, DIA, differentiation inhibitory activity, differentiation stimulating factor, Differentiation-stimulating factor, Emfilermin, HILDAcholinergic differentiation factor, leukemia inhibitory factor cholinergic differentiation factor, leukemia inhibitory factor, LIF, Melanoma-derived LPL inhibitor, MLPLI

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LEIN-L156-25ug-safety-datasheet.pdf