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Anti-Human MMP-13 - 25 µg

ArtNr LEIN-M1275-25ug
Hersteller Leinco Technologies
Menge 25 ug
Quantity options 100 ug 25 ug
Kategorie
Typ Antibody Primary
Applikationen WB, IP, ICC
Specific against Human (Homo sapiens)
Host Caprine
Citations 1. Knäuper, V. et al. (1996) J Biol. Chem. 271: 17124
2. Krane, SM. et al. (2004) National Acad. Scien.
3. Woessner, JF. et al. (2000) J Biol. Chem. 275: 4183
4. Kennedy, AM. et al. (2005) J Clin. Invest. 115: 2832
NCBI 4322
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Versandbedingung Raumtemperatur
Lieferbar
Manufacturer - Applications
Quality Tested by Leinco Western Blotting: To detect Human MMP-13 this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human MMP-13 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:M1242.
Manufacturer - Category
Polyclonal Antibodies>Growth Factors & Cytokines
Manufacturer - Targets
MMP-13
Country of Origin
USA
Shipping Temperature
Next Day Ambient
Storage Conditions
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Product Description
Matrix metallopeptidase 13 (collagenase 3), also known as MMP13, is a glycosylated protein1 and a member of the matrix metalloproteinase (MMP) family of neutral endopeptidases, which is expressed in the skeleton during embryonic development2 and are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. MMP-13 is tightly bound to tissues and utilizes heparan sulfate proteoglycans as extracellular docking molecules. It is produced by many different cell types as an inactive proform that requires proteolytic activation to gain activity.3 Mutations causing lack of MMP-13 expression have been shown to be associated with skeletal disorders characterized by defective growth and modeling of the spine and long bones (spondyloepimetaphyseal dysplasias).4
Background
Matrix metallopeptidase 13 (collagenase 3), also known as MMP13, is a glycosylated protein1 and a member of the matrix metalloproteinase (MMP) family of neutral endopeptidases, which is expressed in the skeleton during embryonic development2 and are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. MMP-13 is tightly bound to tissues and utilizes heparan sulfate proteoglycans as extracellular docking molecules. It is produced by many different cell types as an inactive proform that requires proteolytic activation to gain activity.3 Mutations causing lack of MMP-13 expression have been shown to be associated with skeletal disorders characterized by defective growth and modeling of the spine and long bones (spondyloepimetaphyseal dysplasias).4
Other Applications Reported In Literature
Immunoprecipitation: This antibody has been used to immunoprecipitate recombinant human MMP-13 from conditioned media of transfected NS0 myeloma cells. The recovered recombinant human MMP-13 can be detected by Western blot analysis using monoclonal antibodies.
Immunocytochemistry: This antibody can be used at 5-15 μg/ml to detect human MMP-13 in 10-15 μm thick paraffin-embedded tissue sections.
PubMed
MMP-13
Manufacturer - Specificity
Goat Anti-Human Matrix Metalloproteinase-13 (MMP-13) recognizes Human MMP-13. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
RRID
AB_2831269
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Immunogen
NS0 - Derived Human MMP-13
Additional Information
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords
Polyclonal Antibody, MMP-13, Collagenase 3, CLG3, CL3, MMP-13, Human MMP-13, Anti-Human MMP-13, CLG 3, CLG3EC 3.4.24, collagenase 3, EC 3.4.24.-, EC 3.4.24.22, EC 3.4.24.24, EC 3.4.24.35, EC 3.4.24.65, EC 3.4.24.7, MANDP1, matrix metallopeptidase 13 collagenase 3, matrix metalloproteinase 13 collagenase 3, Matrix metalloproteinase-13, MMP13, MMP-13

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 25 ug
Lieferbar: In stock
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