Anti-Human MMP-8 - 100 µg

Quality Tested by Leinco Western Blotting: To detect Human MMP-8 this polyclonal antibody can be used at a concentration of 0.1 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human MMP-8 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.

MMP-8

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Matrix metallopeptidase 8 (neutrophil collagenase), also known as MMP8, is a part of the MMP family which are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the enzyme encoded by MMP8 is stored in secondary granules within neutrophils and is activated by autolytic cleavage. MMP8 is a neutral metalloproteinase of the fibrillar collagenase family that also includes MMP-1 and MMP-13. In contrast to the other collagenases, MMP-8 has a very limited tissue distribution, thought to be restricted to neutrophils and chondrocytes.1 Its function is degradation of type I, II and III collagens. MMP-8 is the predominant collagenase present in normal healing wounds and suggests that overexpression and activation of this collagenase may be involved in the pathogenesis of nonhealing chronic ulcers.2 MMP-8 might also be useful in monitoring periodontal disease in diabetes.3

Immunoprecipitation: The antibody has been used to immunoprecipitate rhMMP-8 from conditioned media of transfected NS0 cells. The recovered rhMMP-8 can be detected by western blot analysis using a mouse monoclonal antibody.
Immunocytochemistry: The antibodies have been used at 5 μg/mL and 15 μg/mL to detect human MMP-8 in transfected cells.
Immunohistochemistry: Suitable for use at concentration of 5-15 µg/mL.

Goat Anti-Human Matrix Metalloproteinase-8 (MMP-8) recognizes Human MMP-8. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

NS0-Derived Recombinant Human MMP-8

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