Anti-Human NT-3 - 25 µg

IHC FF, N, WB

NT-3

Next Day Ambient

Neurotrophin 3 (NT-3), also known as NTF3 is a basic protein found in neurons of the central nervous system. NT-3 selectively supports the survival of neuronal cell populations. It rapidly potentiate the spontaneous and impulse-evoked synaptic activity of developing neuromuscular synapses in culture and thus appears to be involved in the regulation of functions of developing synapses.1, 2, 3 NT-3 negatively modulates nerve growth factor (NGF) receptor expression and associated nociceptive phenotype in intact neurons, suggesting a beneficial role in treating aspects of neuropathic pain mediated by NGF. NT-3 is effective at suppressing thermal hyperalgesia associated with chronic constriction injury (CCI).4

Immunohistochemistry: Suitable for use at concentration of 5-15 µg/mL.
Neutralization:Measured by its ability to neutralize NT‑3-induced proliferation in BaF‑TrKB-BD mouse pro‑B cell line transfected with TrkB. The Neutralization Dose (ND50) is typically 0.1-0.4 µg/mL in the presence of 20 ng/mL Recombinant Human NT‑3.

Rabbit Anti-Human Neurotrophin-3 (NT-3) recognizes Human NT-3. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

Purified Recombinant Human NT-3 (> 98%)

Polyclonal Antibody, Neurotrophin-3, Nerve Growth Factor-2 (NGF-2), HGN NTF3, HDNF, MGC129711, NT-3, Human NT-3, Anti-Human NT-3, 3-nucleotidase, NT3, NT-3, NTF3