Anti-Mouse Noggin - 100 µg

ICC, IHC FFPE, WB

Noggin

Next Day Ambient

Noggin is a secreted glycoprotein predominantly expressed by the dorsal mesoderm during embryogenesis that inhibits TGF-β signal transduction by binding to TGF-β family ligands and preventing them from binding to their corresponding receptors. Noggin is expressed in condensing cartilage and immature chondrocytes.1 Noggin plays a key role in neural induction by inhibiting BMP4, along with other TGF-β signaling inhibitors such as chordin and follistatin. Noggin plays a crucial role in bone development, joint formation, and neural tube fusion.

IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 5 - 15 µg/ml. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
Immunocytochemistry: Suitable for use at concentration of 5-15 µg/mL.

Goat Anti-Mouse Noggin (Nog) recognizes Mouse Nog. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.