Anti-Human PEDF

ArtNr	LEIN-P266-25ug
Hersteller	Leinco Technologies
Menge	25 ug
Quantity options	100 ug 25 ug
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Applikationen	WB, IHC
Specific against	Human (Homo sapiens)
Host	Caprine
Citations	1. Zhang, SS. et al. (2005) J Struct Biol. 151:130 2. Schwartz, JP. et al. (1998) J Neurosci Res. 53:7 3. Schwartz, JP. et al. (1997) J Neurosci Res. 49:710 4. Kuncl, RW. et al. (1999) J Neuropathol Exp Neurol. 58:719 5. Bouck, NP. et al. (1999) Science 285:245
NCBI	5176
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Versandbedingung	Raumtemperatur
Lieferbar
Manufacturer - Applications	Quality Tested by Leinco Western Blotting: To detect Human PEDF this polyclonal antibody can be used at a concentration of 0.2-2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human PEDF is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:P232.
Manufacturer - Category	Polyclonal Antibodies>Growth Factors & Cytokines
Manufacturer - Targets	PEDF
Country of Origin	USA
Shipping Temperature	Next Day Ambient
Storage Conditions	The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Product Description	Pigment epithelium derived factor (PEDF) is non-inhibitory serpin with neurotrophic and anti-angiogenic functions.1 It protects immature but not mature granule cells against both natural and induced apoptosis.2 PEDF plays an important role in regulation of glial function and proliferation in the central nervous system.3 PEDF also plays important roles in the survival and maintenance of spinal motor neurons in their neuroprotection against acquired insults in postnatal life.4 It is suggested that PEDF may be of therapeutic use, especially in retinopathies where pathological neovascularization compromises vision and leads to blindness.5
Background	Pigment epithelium derived factor (PEDF) is non-inhibitory serpin with neurotrophic and anti-angiogenic functions.¹ It protects immature but not mature granule cells against both natural and induced apoptosis.² PEDF plays an important role in regulation of glial function and proliferation in the central nervous system.³ PEDF also plays important roles in the survival and maintenance of spinal motor neurons in their neuroprotection against acquired insults in postnatal life.⁴ It is suggested that PEDF may be of therapeutic use, especially in retinopathies where pathological neovascularization compromises vision and leads to blindness.⁵
Other Applications Reported In Literature	IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 15 µg/ml. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
PubMed	PEDF
Manufacturer - Specificity	Goat Anti-Human Pigment Epithelium Derived Factor (PEDF) recognizes Human PEDF. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
RRID	AB_2831562
Formulation	This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Endotoxin Level	< 0.1 EU/µg as determined by the LAL method
Immunogen	Purified Recombinant Human PEDF (> 98%)
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

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LEIN-P266-25ug-safety-datasheet.pdf