Anti-Human PDGF Rβ - 25 µg

ELISA Indirect, FC, IHC FFPE, N, WB

PDGF R beta

Next Day Ambient

Platelet-derived growth factor receptor-β (PDGFRβ) is a well-characterized plasma membrane receptor with endogeneous tyrosine kinase activity, which is autophosphorylated in response to binding of dimeric platelet-derived growth factor (PDGF) ligand.1 PDGFRβ mediates signaling important for the proliferation, migration, and survival of mesenchymal cells.2 PDGFRβ is found in all cell types 3 and is activated in breast carcinoma cells and indicate a possible role of the receptor in breast cancerogenesis.4

IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 15 µg/ml. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
Neutralization: The ND₅₀ for anti-human PDGF Rβ antibody was determined to be approximately 0.5-1.5 μg/ml for blocking 120 ng/ml recombinant human PDGF sRβ in the presence of 4 ng/ml of human PDGF-BB on a quiescent confluent culture of NR6R-3T3 cells.
CyTOF-ready:Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Goat Anti-Human Platelet-Derived Growth Factor Receptor Beta (PDGF Rβ) recognizes Human PDGF Rβ. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

Purified Recombinant Human PDGF Rβ (> 98%)

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