Anti-Human CD120b (TNFR2)

Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:T240 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:T408.
Western Blotting: To detect Human TNF RII this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human TNF RII is 0.5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:T408.

TNFR2

Next Day Ambient

Tumor necrosis factor receptor II (TNF-RII) is present on most cell types1 and is considered to play a prominent role in stimulation by TNF-alpha. TNFRII proteins are expressed by hematopoietic cells including macrophages, neutrophils, lymphocytes, thymocytes and mast cells. It is expressed by a variety of other cell types including endothelial cells, cardiac myocytes and prostate cells.2

Neutralization: This antibody is useful for neutralization of Human TNF RII bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human TNF RII (at 0.3 ng/ml) is 0.5 - 1.5 µg/ml.

Mouse Anti-Human Tumor Necrosis Factor Receptor Type II (TNF RII) (Clone 22210) recognizes an epitope on Human TNF RII. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.