Anti-human TSLP

Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 0.8 µg/ml. A suitable detection antibody is PN:B638 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:T142.
Western Blotting: To detect Human TSLP this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as and a chromogenic substrate such as PN:T343. The detection limit for Human TSLP is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:T142.

TSLP

Next Day Ambient

Neutralization: This antibody is useful for neutralization of Human TSLP bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human TSLP (at 0.5 ng/ml) is 0.05-0.25 µg/ml.

Sheep Anti-Human Thymic Stromal Lymphopoietin (TSLP) recognizes Human TSLP. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

Purified Recombinant Human TSLP (> 98%)