Anti-Mouse TER-119 (Erythroid Cells) - Biotin - 500 µg

TER-119

2 - 8°C Wet Ice

TER-119 recognizes a 52 kDa cell surface protein that strongly associates with glycophorin A on the erythrocyte membrane 1 . The antigen is not present on a number of tested cell lines, including T cell, B cell, macrophage, mast cell, fibroblast, epithelial, and murine erythroleukemia cultured lines. TER-119 is a mouse erythroid lineage-specific rat monoclonal antibody that reacts with 100% of mature erythrocytes and 80-90% of day 14 fetal liver cells, after which the percentage of TER-119+ fetal cells declines to 40-50% 2. Additionally, TER-119 stains 20-25% of adult mouse bone marrow cells and 2-3% of spleen cells, but not thymocytes or lymph node cells.TER-119 was produced in Wister rats immunized with BALB/c fetal liver cells 1 . Spleen cells were fused with X63.Ag8.653 myeloma cells to produce a hybridoma clone reactive against fetal liver cells. TER-119 recognizes a 7, 9 di-O-acetyl sialic acid glyco-epitope 3 . Additionally, the epitope is sensitive to hemagglutinin esterase derived from bovine coronavirus (Mebus strain) but not influenza C.

TER-119

Rat Anti-Mouse TER-119 (Clone TER-119) recognizes an epitope on Mouse TER-119. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

0.5 mg/ml

TER-119 is highly specific for erythroid cells, from early proerythroblaststo mature erythrocytes. TER-119 antigen is expressed on normal erythroid cells, but noterythroleukemia cells, even when induced with dimethylsulfoxide. TER-119 is not thought tointeract with pluripotent hematopoietic stem cells in adult bone marrow.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.