« Zurück
Startseite /
Anti-Mouse CD366 (Tim-3) [RMT3-23] – Purified <i>in vivo </i>GOLD™ Functional Grade - 25 mg

Anti-Mouse CD366 (Tim-3) [RMT3-23] – Purified <i>in vivo </i>GOLD™ Functional Grade - 25 mg

ArtNr	LEIN-T700-25mg
Hersteller	Leinco Technologies
Menge	25 mg
Quantity options	1 mg 100 mg 25 mg 5 mg 50 mg
Kategorie	In Vivo Antibodies Flow Cytometry Immunohistochemistry Immunofluorescence Primary Antibodies
Typ	Antibody Monoclonal
Applikationen	FC, IF, IHC, other, IV
Clon	RMT3-23
Specific against	Mouse (Murine, Mus musculus)
Host	Rat
Purity	≥95% monomer by analytical SEC, > 95% by SDS Page
Citations	1. Monney, L. et al. (2002)Nature 415, 536–541. 2. Gao, X. et al. (2012) PLOS ONE 7, e30676. 3. Anderson, A. C. et al. (2007) Science 318, 1141–1143. 4. Ndhlovu, L. C. et al. (2012) Blood 119, 3734–3743. 5. Phong, B. L. et al. J. (2015) Exp. Med. 212, 2289–2304. 6. Li, X. et al. (2010) Clin. Immunol. 134, 169–177. 7. Sanchez-Fueyo, A. et al. (2003) Nat. Immunol. 4, 1093–1101. 8. Zhu C, et al.. Nature Immunology. 2005;6:1245–1252.
NCBI	171285
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Applications	B, FC, IF Staining, IHC, IHC FF, in vivo, PhenoCycler®
Manufacturer - Category	Purified in vivo Functional Grade>Gold Purified Bulk Antibodies\|Primary Monoclonal Antibodies>Immune Checkpoints\|Purified in vivo Functional Grade
Manufacturer - Targets	Tim-3
Country of Origin	USA
Shipping Temperature	Next Day 2 - 8°C
Storage Conditions	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.
Product Description	Tim-3 antibody (clone RMT3-23) recognizes T cell immunoglobulin and mucin domain-containing protein 3 (TIM3, also known as CD366), a TIM family member of immunoregulatory proteins. Tim-3 is a 60kDa type I transmembrane protein with an extracellular immunoglobulin and mucin-like domain and a cytoplasmic tyrosine phosphorylation motif. Tim-3 is expressed by IFNg-producing Th1 CD4 and Tc1 (cytotoxic) CD8 T cells1, regulatory T cells (Tregs)2, myeloid cells3, NK cells4, and mast cells5. Tim-3 is an inhibitory molecule that limits Th1-mediated inflammatory diseases, including a model of central nervous system inflammation (experimental autoimmune encephalomyelitis, EAE)1, inflammatory bowel disease (IBD)6, and type I diabetes7. Tim-3 is also proposed to induce immunological tolerance7, promote Th1 apoptosis8, and regulate macrophage activation1. In addition, Tim-3 is upregulated on tumor-infiltrating lymphocytes, and coblockade of Tim-3 and immune checkpoint inhibitors, such as PD-1, is currently being investigated in clinical trials for the treatment of cancer9.
Background	Tim-3 antibody, clone RMT3-23, recognizes T cell immunoglobulin and mucin domain-containing protein 3 (TIM3, also known as CD366), a TIM family member of immunoregulatory proteins. Tim-3 is a 60kDa type I transmembrane protein with an extracellular immunoglobulin and mucin-like domain and a cytoplasmic tyrosine phosphorylation motif. Tim-3 is expressed by IFNg-producing Th1 CD4 and Tc1 (cytotoxic) CD8 T cells¹, regulatory T cells (Tregs)², myeloid cells³, NK cells⁴, and mast cells⁵. Tim-3 is an inhibitory molecule that limits Th1-mediated inflammatory diseases, including a model of central nervous system inflammation (experimental autoimmune encephalomyelitis, EAE)¹, inflammatory bowel disease (IBD)⁶, and type I diabetes⁷. Tim-3 is also proposed to induce immunological tolerance⁷, promote Th1 apoptosis⁸, and regulate macrophage activation1. In addition, Tim-3 is upregulated on tumor-infiltrating lymphocytes, and coblockade of Tim-3 and immune checkpoint inhibitors, such as PD-1, is currently being investigated in clinical trials for the treatment of cancer⁹.
Function	May play a role in the development of immune responses and the development of Th1-mediated responses
Other Applications Reported In Literature	B IHC
PubMed	Tim-3
Ligand/Receptor	Putative ligand on resting CD4+ lymphocytes
Manufacturer - Research Area	Immunology, Inhibitory Molecules
Manufacturer - Specificity	Clone RMT2-23 recognizes an epitope on mouse Tim-3.
RRID	AB_2737574
Concentration	≥ 5.0 mg/ml
Formulation	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Antigen Distribution	Tim-3 is expressed on activated Th1 and Tc1 lymphocytes, CD11b+ macrophages, Tregs, NK cells, and mast cells.
Immunogen	This antibody was produced using recombinant mouse TIM-3
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords	Monoclonal Antibody, Tim-3, Havcr2, RMT2-23, Mouse, Anti-Mouse Tim-3 - Purified Functional Grade, CD366, FLJ14428, HAVCR2, HAVcr-2, hepatitis A virus cellular receptor 2, kidney injury molecule-3, T cell immunoglobulin mucin 3, T cell immunoglobulin mucin-3, T-cell immunoglobulin and mucin domain-containing protein 3, TIM 3, TIM3 T-cell membrane protein 3, TIM3, TIM-3, TIMD-3, TIMD3KIM-3

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Arbeitsanleitung (Data Sheet) anfordern

Produkt-Datenblatt anfordern

LEIN-T700-25mg-safety-datasheet.pdf