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Anti-Mouse TNFα - Purified in vivo PLATINUM™ Functional Grade

ArtNr LEIN-T703-100mg
Hersteller Leinco Technologies
Menge 100 mg
Quantity options 1 mg 100 mg 25 mg 5 mg 50 mg
Kategorie
Typ Antibody Monoclonal
Applikationen WB, IP, IV
Clon TN3-19.12
Specific against Mouse (Murine, Mus musculus)
Host Hamster - Armenian
Purity > 95% by SDS-PAGE and ≥ 98% monomer by analytical SEC
Citations 1.) El-Harith el-HA et al. (2004) Saudi Med J. 25: 135
2.) Adolf GR et al. (1990) Infec Immun. 58: 3996
3.) Kanneganti et al. (2020) bioRxiv 361048
4.) Fhogartaigh, C.N. et al. (2020) Nat Commun 11, 6385
5.) Karki et al. (2021) Cell. 184:149–168
NCBI 21296
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Applications
Quality Tested by Leinco WB
Manufacturer - Category
Purified in vivo Functional Grade>Platinum Purified Bulk Antibodies|Purified in vivo Functional Grade|Primary Monoclonal Antibodies>Surfacetag Mouse CD Markers
Manufacturer - Targets
TNF-α
Country of Origin
USA
Shipping Temperature
Next Day 2-8°C
Storage Conditions
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Product Description
TNF-α is a 17.5 kD protein that mediates inflammation and immunity caused by the invasion of viruses, bacteria, and parasites by initiating a cascade of cytokines that increase vascular permeability, thus bringing macrophages and neutrophils to the site of infection. TNF-α secreted by the macrophage causes the blood to clot which provides containment of the infection. TNF-α binding to surface receptors brings about various biologic activities that include cytolysis and cytostasis of many tumor cell lines In vitro, hemorraghic necrosis of tumors In vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.
Background
TNF-α is a 17.5 kD protein that mediates inflammation and immunity caused by the invasion of viruses, bacteria, and parasites by initiating a cascade of cytokines that increase vascular permeability, thus bringing macrophages and neutrophils to the site of infection. TNF-α secreted by the macrophage causes the blood to clot which provides containment of the infection. TNF-α binding to surface receptors brings about various biologic activities that include cytolysis and cytostasis of many tumor cell lines In vitro, hemorraghic necrosis of tumors In vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Additional Reported Applications For Relevant Conjugates
N
For specific conjugates of this clone, review literature for suggested application details.
Other Applications Reported In Literature
IP
PubMed
TNF-α
Ligand/Receptor
TNFRSF1A (TNF-R1, CD120a, TNFR-p60 Type β, p55); TNFRSF1B (TNF-R2, CD120b, TNFR-p80 Type A, p75)
Manufacturer - Specificity
Clone TN3-19.12 recognizes mouse TNFα.
RRID
AB_2832119
Concentration
2.0 mg/ml
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Antigen Distribution
TNF-α is secreted by macrophages, monocytes, neutrophils, T cells, B cells, NK cells, LAK cells.
Endotoxin Level
≤ 0.5 EU/mg
Immunogen
Purified Recombinant Mouse TNF-α
Additional Information
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 mg
Lieferbar: In stock
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