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Anti-Mouse VE-Cadherin - 100 µg

ArtNr LEIN-V172-100ug
Hersteller Leinco Technologies
Menge 100 ug
Quantity options 100 ug 25 ug
Kategorie
Typ Antibody Monoclonal
Applikationen WB, FC
Clon 162709
Specific against Mouse (Murine, Mus musculus)
Host Rat
NCBI 12562
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Versandbedingung Raumtemperatur
Lieferbar
Manufacturer - Applications
Quality Tested by Leinco Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing VE-Cadherin. A suggested method would be to stain cells expressing VE-Cadherin with 0.25 µg/106 cells in a total staining volume of 200 µl followed by PN:R485.
Western Blotting: To detect Mouse VE-Cadherin this monoclonal antibody can be used at a concentration of 1 - 2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse VE-Cadherin is 5 - 50 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Manufacturer - Category
Primary Monoclonal Antibodies>Growth Factors and Cytokines
Manufacturer - Targets
VE-Cadherin
Country of Origin
USA
Shipping Temperature
Next Day Ambient
Storage Conditions
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
PubMed
VE-Cadherin
Manufacturer - Specificity
Rat Anti-Mouse Vascular endothelial-Cadherin (VE-Cadherin) (Clone 162709) recognizes an epitope on Mouse VE-Cadherin. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
RRID
AB_2832170
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Immunogen
Purified Recombinant Mouse VE-Cadherin (> 98%)
Additional Information
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords
Monoclonal Antibody, Vascular endothelial-Cadherin, Clone 162709, VE-Cadherin, Mouse VE-Cadherin, Anti-Mouse VE-Cadherin, 7B4 antigen, 7B4, cadherin 5, type 2 (vascular endothelium), cadherin 5, type 2, VE-cadherin (vascular epithelium), Cadherin-5, CD144 antigen, CD144, CDH5, endothelial-specific cadherin, FLJ17376, Vascular endothelial cadherin, VECadherin, VE-Cadherin

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 ug
Lieferbar: In stock
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