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ACE2 Antibody [3F1]

ACE2 Antibody [3F1]

Hersteller	ProSci
Kategorie	Oncology Neuroscience Aging & Neurological Disorders Infectious Diseases Viral Infections Coronavirus (COVID-19) Antibodies Immunohistochemistry Western Blot Immunofluorescence Immunocytochemistry Cardiovascular Research Heart Failure Myocardial Infarction Primary Antibodies By Organ Colorectal Cancer Testicular & Prostate Cancer Gastric Cancer Uterine, Ovarian & Cervical Cancer
Typ	Antibody Monoclonal
Specific against	other
Format	Liquid
Applikationen	WB, IF, IHC
Menge	100 ul
Host	Rabbit
ArtNr	PRS-10-604-100ul
eClass 6.1	32160702
eClass 9.0	42030590
Lieferbar
Shipping	blue ice or RT
By Research Area	Infectious Disease
Immunogen	C terminal human Angiotensin Converting Enzyme 2
Applications	WB(1:300-1000), IF(ICC)(1:50-200), IHC()
Purification	Purified by Protein A.
Clonality	Monoclonal
Clone	3F1
Isotype	IgG
Conjugate	Unconjugated
Buffer	1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide
Concentration	1 mg/mL
Storage Conditions	Store at 4˚ C short term. Aliquot and store at -20˚ C long term. Avoid freeze-thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	ACE2
Accession #	Q9BYF1
Ncbi Gene Id #	59272
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	ACE2
Ncbi Official Full Name	Angiotensin-converting enzyme 2
Ncbi Organism	Homo sapiens
Swissprot #	Q9BYF1
Background	Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses.
1st Image Caption	Figure 1 Western Blot Validation of ACE2 Human kidney probed with ACE2 antibody [3F1], 10-604, at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
2nd Image Caption	Figure 2 Immunofluorescence Validation of ACE2 in MCF-7 cells IF (ICC) staining with ACE2 antibody [3F1], 10-604, at 1:300 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
3rd Image Caption	Figure 3 Immunofluorescence Validation of ACE2 in HepG2 cells IF (ICC) staining with ACE2 antibody [3F1], 10-604, at 1:300 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
4th Image Caption	Figure 4 Immunohistochemistry Validation of ACE2 in Human Breast Carcinoma Tissue Paraformaldehyde-fixed and paraffin-embedded human breast carcinoma tissue incubated with ACE2 antibody [3F1], 10-604, at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
5th Image Caption	Figure 5 Immunohistochemistry Validation of ACE2 in Human Kidney Tissue Paraformaldehyde-fixed and paraffin-embedded human kidney tissue incubated with ACE2 antibody [3F1], 10-604, at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
6th Image Caption	Figure 6 Immunohistochemistry Validation of ACE2 in Mouse Kidney Tissue Paraformaldehyde-fixed and paraffin-embedded mouse kidney tissue incubated with ACE2 antibody [3F1], 10-604, at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 100 ul

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Lieferung vsl. bis 06.06.2024

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