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ACE2 Antibody [3F1]

ACE2 Antibody [3F1]

Manufacturer	ProSci
Category	Oncology Neuroscience Aging & Neurological Disorders Infectious Diseases Viral Infections Coronavirus (COVID-19) Antibodies Immunohistochemistry Western Blot Immunofluorescence Immunocytochemistry Cardiovascular Research Heart Failure Myocardial Infarction Primary Antibodies By Organ Colorectal Cancer Testicular & Prostate Cancer Gastric Cancer Uterine, Ovarian & Cervical Cancer
Type	Antibody Monoclonal
Specific against	other
Format	Liquid
Applications	WB, IF, IHC
Amount	100 ul
Host	Rabbit
Item no.	PRS-10-604-100ul
eClass 6.1	32160702
eClass 9.0	42030590
Available
Shipping	blue ice or RT
By Research Area	Infectious Disease
Immunogen	C terminal human Angiotensin Converting Enzyme 2
Applications	WB(1:300-1000), IF(ICC)(1:50-200), IHC()
Purification	Purified by Protein A.
Clonality	Monoclonal
Clone	3F1
Isotype	IgG
Conjugate	Unconjugated
Buffer	1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide
Concentration	1 mg/mL
Storage Conditions	Store at 4˚ C short term. Aliquot and store at -20˚ C long term. Avoid freeze-thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	ACE2
Accession #	Q9BYF1
Ncbi Gene Id #	59272
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	ACE2
Ncbi Official Full Name	Angiotensin-converting enzyme 2
Ncbi Organism	Homo sapiens
Swissprot #	Q9BYF1
Background	Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses.
1st Image Caption	Figure 1 Western Blot Validation of ACE2 Human kidney probed with ACE2 antibody [3F1], 10-604, at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
2nd Image Caption	Figure 2 Immunofluorescence Validation of ACE2 in MCF-7 cells IF (ICC) staining with ACE2 antibody [3F1], 10-604, at 1:300 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
3rd Image Caption	Figure 3 Immunofluorescence Validation of ACE2 in HepG2 cells IF (ICC) staining with ACE2 antibody [3F1], 10-604, at 1:300 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
4th Image Caption	Figure 4 Immunohistochemistry Validation of ACE2 in Human Breast Carcinoma Tissue Paraformaldehyde-fixed and paraffin-embedded human breast carcinoma tissue incubated with ACE2 antibody [3F1], 10-604, at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
5th Image Caption	Figure 5 Immunohistochemistry Validation of ACE2 in Human Kidney Tissue Paraformaldehyde-fixed and paraffin-embedded human kidney tissue incubated with ACE2 antibody [3F1], 10-604, at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
6th Image Caption	Figure 6 Immunohistochemistry Validation of ACE2 in Mouse Kidney Tissue Paraformaldehyde-fixed and paraffin-embedded mouse kidney tissue incubated with ACE2 antibody [3F1], 10-604, at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ul

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Delivery expected until 6/6/2024