GAPDH antibody

ArtNr	NKM-ATGA0559-50ul
Hersteller	NKMAX
Menge	50 ul
Quantity options	100 ul 50 ul
Kategorie	Neuroscience Metabolism Regulation of Appetite Protein Aggregation, Clearance & Misfolding Antibodies Western Blot ELISA Immunofluorescence Immunocytochemistry Primary Antibodies Immunostaining Immunochemistry
Typ	Antibody Monoclonal
Format	Liquid
Applikationen	WB, ICC, ELISA
Clon	AT4E8
Specific against	other
Host	Mouse
Isotype	IgG2b Kappa
Konjugat/Tag	Unconjugated
Citations	Chuang DM., et al, (2005) Annu Rev Pharmacol Toxicol. 45:269-90. Mazzola JL., et al, (2002) Neurotoxicology. 23(4-5):603-9. Sirover MA. (1997) J Cell Biochem. 66(2):133-40. Chang Y, et al. Clinical impact of serum exosomal microRNA in liver fibrosis. (PLoS One. 2021) Hah YS, et al. β-Sitosterol Attenuates Dexamethasone-Induced Muscle Atrophy via Regulating FoxO1-Dependent Signaling in C2C12 Cell and Mice Model. (Nutrients. 2022) Hah YS, et al. Rutin Prevents Dexamethasone-Induced Muscle Loss in C2C12 Myotube and Mouse Model by Controlling FOXO3-Dependent Signaling. (Antioxidants. 2023) Park SW, et al. Development of new tools to study membrane-anchored mammalian Atg8 proteins. (Autophagy. 2023)
NCBI	NP_001075722.1
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	Glyceraldehyde-3-phosphate dehydrogenase isoform 1,Peptidyl-cysteine S-nitrosylase GAPDH,GAPD,G3PD
Lieferbar
Manufacturer - Type	Antibodies
Manufacturer - Applications	ELISA, WB, ICC/IF
Manufacturer - Category	Monoclonal antibody
Storage Conditions	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Description	Multiple roles for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) have been recently appreciated. GAPDH is found in the particulate fractions, such as the nucleus, the mitochondria, and the small vesicular fractions. GAPDH gene expression is specifically increased during programmed neuronal cell death. When cells are exposed to various stressors, dynamic subcellular re-distribution of GAPDH occurs. GAPDH is also involved in various diseases, especially neurodegenerative disorders and cancers. As a membrane protein, GAPDH functions in endocytosis in the cytoplasm, it is involved in the translational control of gene expression in the nucleus, it functions in nuclear tRNA export, in DNA replication, and in DNA repair.
Concentration	1mg/ml (determined by BCA assay)
Formulation	Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen	GAPDH from rabbit muscle
Ab Usage	The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Antigen Species	N/A
Clonality	Monoclonal
Ab Purification Comment	By protein-A affinity chromatography
Gene Category	Cancer
Additional Comment Only	This product was produced from tissue culture supernatant.
Manufacturer - Search Terms	wu:fb33a10, Peptidyl-cysteine S-nitrosylase GAPDH, Peptidyl cysteine S nitrosylase GAPDH, p38 component, OTTHuMP00000174432, OTTHuMP00000174431, OCT1 coactivator in S phase, OCAS, MGC88685, MGC127711, MGC105239, MGC103191, MGC103190, MGC102546, MGC102544, KNC-NDS6, KNC NDS6, HEL-S-162eP, HEL S 162eP, Glyceraldehyde-3-phosphate dehydrogenase isoform 2, Glyceraldehyde-3-phosphate dehydrogenase isoform 1, Glyceraldehyde-3-phosphate dehydrogenase 38 kDa BFA-dependent ADP-ribosylation substrate, Glyceraldehyde-3-phosphate dehydrogenase, Glyceraldehyde3 phosphate dehydrogenase, Glyceraldehyde 3 phosphate dehydrogenase isoform 2, Glyceraldehyde 3 phosphate dehydrogenase isoform 1, Glyceraldehyde 3 phosphate dehydrogenase 38 kDa BFA dependent ADP ribosylation substrate, Glyceraldehyde 3 phosphate dehydrogenase, GAPDH human, GAPDH, GAPD, G3PDH, G3PD, EC1.2.1.12, EC 1.2.1.12, cb609, BARS-38, BARS 38, Aging-associated gene 9 protein, Aging associated gene 9 protein, 38-KD component, 38 KD compone
Manufacturer - Similar Names	wu:fb33a10, Peptidyl-cysteine S-nitrosylase GAPDH, Peptidyl cysteine S nitrosylase GAPDH, p38 component, OTTHuMP00000174432, OTTHuMP00000174431, OCT1 coactivator in S phase, OCAS, MGC88685, MGC127711, MGC105239, MGC103191, MGC103190, MGC102546, MGC102544, KNC-NDS6, KNC NDS6, HEL-S-162eP, HEL S 162eP, Glyceraldehyde-3-phosphate dehydrogenase isoform 2, Glyceraldehyde-3-phosphate dehydrogenase isoform 1, Glyceraldehyde-3-phosphate dehydrogenase 38 kDa BFA-dependent ADP-ribosylation substrate, Glyceraldehyde-3-phosphate dehydrogenase, Glyceraldehyde3 phosphate dehydrogenase, Glyceraldehyde 3 phosphate dehydrogenase isoform 2, Glyceraldehyde 3 phosphate dehydrogenase isoform 1, Glyceraldehyde 3 phosphate dehydrogenase 38 kDa BFA dependent ADP ribosylation substrate, Glyceraldehyde 3 phosphate dehydrogenase, GAPDH human, GAPDH, GAPD, G3PDH, G3PD, EC1.2.1.12, EC 1.2.1.12, cb609, BARS-38, BARS 38, Aging-associated gene 9 protein, Aging associated gene 9 protein, 38-KD component, 38 KD compone
IF Comment	ICC/IF analysis of GAPDH in HeLa cells. The cell was stained with ATGA0559 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
WB Comment	The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with ant-GAPDH antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HepG2 cell lysate Lane 2.: MCF7 cell lysate Lane 3.: Jurkat cell lysate Lane 4.: K562 cell lysate Lane 5.: A431 cell lysate Lane 6.: LNCap cell lysate Lane 7.: Ramos cell lysateThe tissue lysates (20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probedwith anti-GAPDH antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Mouse Brain tissue lysate Lane 2.: Mouse Spleen tissue lysate Lane 3.: Mouse Eye tissue lysate Lane 4.: Mouse Muscle tissue lysate The 293T cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-GAPDH. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRPand an ECL detection system. Lane 1.: Anti-GAPDH monoclonal antibody (1:100, 000) Lane 2.: Anti-GAPDH monoclonal antibody (1:50, 000) Lane 3.: Anti-GAPDH monoclonal antibody (1:10, 000) Lane 4.: Anti-GAPDH monoclonal antibody (1:1, 000)

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