IRAK Antibody

Independent Antibody Validation in Cell lines (Figure 2) shows similar IRAK expression profile in human cell lines detected by two independent anti-IRAK antibodies that recognize different epitopes, 1007 against C-terminus domain and 64-231 against another region of C-terminus domain.  IRAK proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies.

Recombinant Protein Test (Figure 3): Anti-IRAK antibodies (1007) detected human IRAK recombinant protein at different concentrations.

Immunoprecipitation validation (Figure 6): IRAK1 protein and pellino protein were immunoprecipitated by anti-IRAK antibodies (1007) in HEK293T cells with co-expression of Pellino proteins and IRAK-1.

Overexpression validation (Figure 7): IRAK1 overexpression in 293T cells was detected by anit-IRAK antibodies (1007).

KD validation (Figure 8): Anti-IRAK antibody (1007) specificity was further verified by IRAK specific knockdown. IRAK signal in human chondrocytes transfected with IRAK siRNAs was disrupted in comparison with that in cells transfected with control siRNAs.