PUMA Antibody

Blue Ice

Predicted: 21kD
Observed: 23kD

PUMA Antibody: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53 (1). The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta (2). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.

Trial Size, IAV, KO, Cell Treatment, Most Cited, Most Images

15193488

Human PUMA has 4 isoforms, including isoform 1 (193aa, 21kD), isoform 2 (131aa, 14kD), isoform 3 (101aa, 10kD) and isofrom 4 (261aa, 27kD). This antibody detects human isoform 1, and is predicted to detect mouse and rat PUMA (193aa, 21kD for both of them).

PRS-1210-0.1mg

PRS-10-001-4um

1 mg/mL

WB: 2-3 μ g/mL; IF: 10-20 μ g/mL; IHC: 2.5-10 μ g/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.

Anti-PUMA antibody (3043) was raised against a peptide corresponding to 14 amino acids near the amino terminus of human PUMA isoform 1.

The immunogen is located within the first 50 amino acids of PUMA.

BCL2 binding component 3

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.