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Neurofilament Antibody [NF421]

Neurofilament Antibody [NF421]

ArtNr	PRS-33-179-100ug
Hersteller	ProSci
Menge	100 ug
Kategorie	Neuroscience Neural Lineage Markers Molecular Targets for Neuroscience Antibodies Flow Cytometry Immunohistochemistry Western Blot Primary Antibodies Neurodegeneration Markers Glial Cell Markers
Typ	Antibody Monoclonal
Format	Liquid
Applikationen	WB, FC, IHC
Clon	NF421
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus), Chicken (Gallus gallus domesticus), Pig (Porcine, Sus scrofa domesticus)
Host	Mouse
Isotype	IgG1
Konjugat/Tag	Unconjugated
NCBI	NEFH
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	NEFH, 200 kDa neurofilament protein, KIAA0845, NFH, NF-H
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Manufacturer - Applications	Flow Cytometry: 0.5-1 ug/million cells IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (2) Prediluted format : incubate for 30 min at RT (3) The usable concentrations in the Applications Details are suggestions only. The use of different protocols/secondaries/substrates may require the Neurofilament antibody to be titered for optimal performance. 1. The NF421 antibody is specific to the heavy subunit. 2. FFPE tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Shipping Temperature	blue ice or room temperature
Storage Conditions	Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Manufacturer - Research Area	Neuroscience, Signal Transduction, Stem Cell
Background	Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). The fourth neurofilament subunit is alpha internexin in the central nervous system and peripherin in the peripheral nervous system.
Purification	Protein G purified Neurofilament antibody
Concentration	0.2 mg/mL
Buffer	PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Immunogen	Human recombinant Neurofilament protein was used as the immunogen. (1)
Clonality	Monoclonal
NCBI Official Name	neurofilament, heavy polypeptide
NCBI Organism	Homo sapiens
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 100 ug

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Lieferung vsl. bis 06.11.2025

Express-Lieferung: 27 Tage sparen

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