« Back
Home /
Neurofilament Antibody [NF421]

Neurofilament Antibody [NF421]

Item no.	PRS-33-179-100ug
Manufacturer	ProSci
Amount	100 ug
Category	Neuroscience Neural Lineage Markers Molecular Targets for Neuroscience Antibodies Flow Cytometry Immunohistochemistry Western Blot Primary Antibodies Neurodegeneration Markers Glial Cell Markers
Type	Antibody Monoclonal
Format	Liquid
Applications	WB, FC, IHC
Clone	NF421
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus), Chicken (Gallus gallus domesticus), Pig (Porcine, Sus scrofa domesticus)
Host	Mouse
Isotype	IgG1
Conjugate/Tag	Unconjugated
NCBI	NEFH
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	NEFH, 200 kDa neurofilament protein, KIAA0845, NFH, NF-H
Available
Manufacturer - Applications	Flow Cytometry: 0.5-1 ug/million cells IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (2) Prediluted format : incubate for 30 min at RT (3) The usable concentrations in the Applications Details are suggestions only. The use of different protocols/secondaries/substrates may require the Neurofilament antibody to be titered for optimal performance. 1. The NF421 antibody is specific to the heavy subunit. 2. FFPE tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Shipping Temperature	blue ice or room temperature
Storage Conditions	Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Manufacturer - Research Area	Neuroscience, Signal Transduction, Stem Cell
Background	Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). The fourth neurofilament subunit is alpha internexin in the central nervous system and peripherin in the peripheral nervous system.
Purification	Protein G purified Neurofilament antibody
Concentration	0.2 mg/mL
Buffer	PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Immunogen	Human recombinant Neurofilament protein was used as the immunogen. (1)
Clonality	Monoclonal
NCBI Official Name	neurofilament, heavy polypeptide
NCBI Organism	Homo sapiens
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

Request product datasheet

Request safety datasheet

Simple Order Order Subscription

Amount: 100 ug

available

Delivery expected until 11/6/2025

Express Delivery: Save 27 days

Compare

Add to wishlist

Get an offer