Vergleich

CD45RB Antibody [PD7/26]

ArtNr PRS-33-196-100ug
Hersteller ProSci
Menge 100 ug
Kategorie
Typ Antibody Monoclonal
Format Liquid
Applikationen WB, FC, IF, IHC
Clon PD7/26
Specific against Human (Homo sapiens)
Host Mouse
Isotype IgG1 Kappa
Konjugat/Tag Unconjugated
NCBI PTPRC
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Alias Receptor-type tyrosine-protein phosphatase C,Leukocyte common antigen,L-CA,T200,CD45,PTPRC,CD45
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
Primary Antibodies
Shipping Temperature
Blue Ice
Storage Conditions
Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Manufacturer - Research Area
Cancer, Immunology, Neuroscience, Signal Transduction
Background
CD45RB is one of the four major isoforms of CD45.  The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved.  Clone PD7/26 antibody is specific to CD45RB, and stains most leukemias and lymphomas. This CD45RB antibody is often used in a cocktail with clone 2B11, which will bind to all CD45 isoforms (Cat No V2243).
By Type
Cancer, Immunology, Neuroscience, Signal Transduction
Modifications
None
Purification
Protein G purified
Concentration
0.2 mg/mL
Buffer
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Application Note
Western Blot: 0.5-1 ug/ml
Flow Cytometry: 0.5-1 ug/million cells
IF: 0.5-1 ug/ml
IHC (FFPE): 0.5-1.0 ug/ml for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
Variations in protocols, secondaries and substrates may require the CD45RB antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen
Human peripheral blood lymphocytes maintained in T cell growth factor were used as the immunogen for this CD45RB antibody.
Clonality
Monoclonal
NCBI Official Name
protein tyrosine phosphatase, receptor type, C
NCBI Organism
Homo sapiens
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
User Note
Optimal dilutions for each application to be determined by the researcher

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 ug
Lieferbar: In stock
lieferbar

Vergleichen

Auf den Wunschzettel

Angebot anfordern

Lieferzeit anfragen

Technische Frage stellen

Bulk-Anfrage stellen

Fragen zum Produkt?
 
Schließen