Comparison

CD45RB Antibody [PD7/26]

Item no. PRS-33-196-100ug
Manufacturer ProSci
Amount 100 ug
Category
Type Antibody Monoclonal
Format Liquid
Applications WB, FC, IF, IHC
Clone PD7/26
Specific against Human (Homo sapiens)
Host Mouse
Isotype IgG1 Kappa
Conjugate/Tag Unconjugated
NCBI PTPRC
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Alias Receptor-type tyrosine-protein phosphatase C,Leukocyte common antigen,L-CA,T200,CD45,PTPRC,CD45
Shipping Condition Cool pack
Available
Manufacturer - Type
Primary Antibodies
Shipping Temperature
Blue Ice
Storage Conditions
Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Manufacturer - Research Area
Cancer, Immunology, Neuroscience, Signal Transduction
Background
CD45RB is one of the four major isoforms of CD45.  The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved.  Clone PD7/26 antibody is specific to CD45RB, and stains most leukemias and lymphomas. This CD45RB antibody is often used in a cocktail with clone 2B11, which will bind to all CD45 isoforms (Cat No V2243).
By Type
Cancer, Immunology, Neuroscience, Signal Transduction
Modifications
None
Purification
Protein G purified
Concentration
0.2 mg/mL
Buffer
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Application Note
Western Blot: 0.5-1 ug/ml
Flow Cytometry: 0.5-1 ug/million cells
IF: 0.5-1 ug/ml
IHC (FFPE): 0.5-1.0 ug/ml for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
Variations in protocols, secondaries and substrates may require the CD45RB antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen
Human peripheral blood lymphocytes maintained in T cell growth factor were used as the immunogen for this CD45RB antibody.
Clonality
Monoclonal
NCBI Official Name
protein tyrosine phosphatase, receptor type, C
NCBI Organism
Homo sapiens
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
User Note
Optimal dilutions for each application to be determined by the researcher

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 ug
Available: In stock
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