Vergleich

HER-2 Antibody [ERB2/776]

ArtNr PRS-33-272-100ug
Hersteller ProSci
Menge 100 ug
Kategorie
Typ Antibody Monoclonal
Format Liquid
Applikationen WB, FC, IF, IHC
Clon ERB2/776
Specific against Human (Homo sapiens)
Host Mouse
Isotype IgG1 Kappa
Konjugat/Tag Unconjugated
NCBI Her2
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Alias erbB-2 Antibody: NEU,NGL,HER2,TKR1,CD340,HER-2,MLN 19,HER-2/neu,MLN19,NEU,Receptor tyrosine-protein kinase erbB-2,Metastatic lymph node gene 19 protein
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
Primary Antibodies
Shipping Temperature
Blue Ice
Storage Conditions
Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Manufacturer - Research Area
Cancer
Background
ErbB2, also called HER2, is a member of the EGFR family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. This ErbB2 antibody shows minimal cross-reaction with other members of the family. The protein is over-expressed in a variety of carcinomas especially those of breast and ovary.
By Type
Cancer
Modifications
None
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Buffer
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Application Note
Flow Cytometry: 0.5-1 ug/million cells
IF: 0.5-1 ug/ml
IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HER-2 antibody to be titered up or down for optimal performance.
1. FFPE staining requires boiling tissue sections in 10mM Citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
2. This HER2 antibody is specific to the intracellular region of the protein.
Immunogen
Recombinant human protein (intracellular domain) was used as the immunogen for this HER-2 antibody. (2)
Clonality
Monoclonal
NCBI Official Name
erb-b2 receptor tyrosine kinase 2
NCBI Organism
Homo sapiens
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
User Note
Optimal dilutions for each application to be determined by the researcher

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 ug
Lieferbar: In stock
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