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HER-2 Antibody [ERB2/776]

HER-2 Antibody [ERB2/776]

Item no.	PRS-33-272-100ug
Manufacturer	ProSci
Amount	100 ug
Category	Oncology Neuroscience Neural Adhesion Molecules Antibodies By Cell Type Blastoma Carcinoma Glioma Hematology Leukemia Lymphoma Myeloma Melanoma Sarcoma Flow Cytometry Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies By Organ Lung Cancer Bladder Cancer Colorectal Cancer Breast Cancer
Type	Antibody Monoclonal
Format	Liquid
Applications	WB, FC, IF, IHC
Clone	ERB2/776
Specific against	Human (Homo sapiens)
Host	Mouse
Isotype	IgG1 Kappa
Conjugate/Tag	Unconjugated
NCBI	Her2
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	erbB-2 Antibody: NEU,NGL,HER2,TKR1,CD340,HER-2,MLN 19,HER-2/neu,MLN19,NEU,Receptor tyrosine-protein kinase erbB-2,Metastatic lymph node gene 19 protein
Shipping Condition	Cool pack
Available
Manufacturer - Type	Primary Antibodies
Shipping Temperature	Blue Ice
Storage Conditions	Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Manufacturer - Research Area	Cancer
Background	ErbB2, also called HER2, is a member of the EGFR family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. This ErbB2 antibody shows minimal cross-reaction with other members of the family. The protein is over-expressed in a variety of carcinomas especially those of breast and ovary.
By Type	Cancer
Modifications	None
Purification	Protein G affinity chromatography
Concentration	0.2 mg/mL
Buffer	PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Application Note	Flow Cytometry: 0.5-1 ug/million cells IF: 0.5-1 ug/ml IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1) The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HER-2 antibody to be titered up or down for optimal performance. 1. FFPE staining requires boiling tissue sections in 10mM Citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min. 2. This HER2 antibody is specific to the intracellular region of the protein.
Immunogen	Recombinant human protein (intracellular domain) was used as the immunogen for this HER-2 antibody. (2)
Clonality	Monoclonal
NCBI Official Name	erb-b2 receptor tyrosine kinase 2
NCBI Organism	Homo sapiens
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
User Note	Optimal dilutions for each application to be determined by the researcher

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ug

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Delivery expected until 12/4/2025

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