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GRP94 Antibody [SRPR90b]

ArtNr PRS-34-004-100ug
Hersteller ProSci
Menge 100 ug
Kategorie
Typ Antibody Monoclonal
Format Liquid
Applikationen WB, IF, IHC-P
Clon SRPR90b
Specific against Human (Homo sapiens)
Host Rat
Isotype IgG2a Kappa
Konjugat/Tag Unconjugated
NCBI HSP90B1
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Alias Endoplasmin,94 kDa glucose-regulated protein,GRP-94,Heat shock protein 90 kDa beta member 1,Tumor rejection antigen 1,gp96 homolog,HSP90B1,GRP94,TRA1
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
Primary Antibodies
Shipping Temperature
Blue Ice
Storage Conditions
Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Manufacturer - Research Area
Cancer, Obesity, Signal Transduction
Background
Heat shock protein 90kDa beta member 1, known also as endoplasmin, gp96, grp94 and ERp99, is an HSP90 paralogue that is found in the endoplasmic reticulum. It plays critical roles in folding proteins in the secretory pathway such as Toll-like receptors and integrins. It has been implicated as an essential immune chaperone to regulate both innate and adaptive immunity. [Wiki]
By Type
Cancer, Obesity, Signal Transduction
Modifications
None
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Buffer
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Application Note
Western blot: 1-2 ug/ml
Immunofluorescence: 1-2 ug/ml
Immunohistology (FFPE): 1-2 ug/ml for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)
Titering of the GRP94 antibody may be required for optimal performance.

1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen
Recombinant full-length human HSP90B1 protein was used as the immunogen for the GRP94 antibody.
Clonality
Monoclonal
NCBI Official Name
heat shock protein 90kDa beta (Grp94), member 1
NCBI Organism
Homo sapiens
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
User Note
Optimal dilutions for each application to be determined by the researcher

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 ug
Lieferbar: In stock
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