Vergleich

TGF-alpha Antibody [SPM542]

ArtNr PRS-34-170-100ug
Hersteller ProSci
Menge 100 ug
Kategorie
Typ Antibody Monoclonal
Format Liquid
Applikationen FC, IF, IHC
Clon SPM542
Specific against Human (Homo sapiens), Rabbit (Oryctolagus cuniculus), Zebrafish (Danio rerio)
Host Mouse
Isotype IgM Kappa
Konjugat/Tag Unconjugated
NCBI TGFA
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Alias TFGAProtransforming growth factor alpha,EGF-like TGF,TGF-alpha
Versandbedingung Gekühlt
Lieferbar
Specificity Human, Rabbit, Zebrafish
Manufacturer - Type
Primary Antibodies, Functional Antibodies, Antibody Pairs
Manufacturer - Isotype
IgM, kappa
Shipping Temperature
Blue Ice
Storage Conditions
Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Manufacturer - Research Area
Growth Factor, Antibody Pairs, Functional Assays
Background
This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. TGFa is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.
By Type
Growth Factors
Modifications
None
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Buffer
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Application Note
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 1-2 ug/ml
Immunohistochemistry (FFPE): 2-4 ug/ml for 30 min at RT (1)
The optimal dilution of the anti-TGF-alpha antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Immunogen
Amino acids 34-43 (PPVAAAVVSH) from the human protein were used as the immunogen for this anti-TGF alpha antibody.
Clonality
Monoclonal
NCBI Official Name
transforming growth factor, alpha
NCBI Organism
Homo sapiens
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
User Note
Optimal dilutions for each application to be determined by the researcher

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 ug
Lieferbar: In stock
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