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TGF-alpha Antibody [SPM542]

TGF-alpha Antibody [SPM542]

Item no.	PRS-34-170-100ug
Manufacturer	ProSci
Amount	100 ug
Category	Oncology Neuroscience Neural Signaling Antibodies By Cell Type Blastoma Carcinoma Hematology Leukemia Lymphoma Myeloma Cell Biology Cell Status Apoptosis Flow Cytometry Immunohistochemistry Immunofluorescence Autophagy Cardiovascular Research Ischemia & Reperfusion Injury Primary Antibodies By Organ Colorectal Cancer Testicular & Prostate Cancer
Type	Antibody Monoclonal
Format	Liquid
Applications	FC, IF, IHC
Clone	SPM542
Specific against	Human (Homo sapiens), Rabbit (Oryctolagus cuniculus), Zebrafish (Danio rerio)
Host	Mouse
Isotype	IgM Kappa
Conjugate/Tag	Unconjugated
NCBI	TGFA
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	TFGAProtransforming growth factor alpha,EGF-like TGF,TGF-alpha
Shipping Condition	Cool pack
Available
Specificity	Human, Rabbit, Zebrafish
Manufacturer - Type	Primary Antibodies, Functional Antibodies, Antibody Pairs
Manufacturer - Isotype	IgM, kappa
Shipping Temperature	Blue Ice
Storage Conditions	Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Manufacturer - Research Area	Growth Factor, Antibody Pairs, Functional Assays
Background	This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. TGFa is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.
By Type	Growth Factors
Modifications	None
Purification	Protein G affinity chromatography
Concentration	0.2 mg/mL
Buffer	PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Application Note	Flow Cytometry: 0.5-1 ug/million cells in 0.1ml Immunofluorescence: 1-2 ug/ml Immunohistochemistry (FFPE): 2-4 ug/ml for 30 min at RT (1) The optimal dilution of the anti-TGF-alpha antibody for each application should be determined by the researcher. 1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Immunogen	Amino acids 34-43 (PPVAAAVVSH) from the human protein were used as the immunogen for this anti-TGF alpha antibody.
Clonality	Monoclonal
NCBI Official Name	transforming growth factor, alpha
NCBI Organism	Homo sapiens
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
User Note	Optimal dilutions for each application to be determined by the researcher

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ug

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Delivery expected until 11/6/2025

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