IDO Antibody

ELISA: (direct and indirect: 1:2, 000-1:10, 000). Immunocytochemistry: (1:500-1:2, 000). Immunohistochemistry: (paraffin sections (1:200-1:500)). Western Blot: (1:2, 000-1:5, 000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.

Stable for at least 6 months after receipt when stored at -20°C.

IDO catalyzes the first and rate-limiting step in the main pathway of human tryptophan catabolism, the kynurenine pathway. Proinflammatory mediators, such as endotoxin and IFN-gamma induce the expression of IDO in several tissues. IDO-dependent suppression of T-cell responses might function as natural immunoregulatory mechanism. Physiological IDO activity has been implicated in T-cell tolerance to tumors, dysfunctional selftolerance in non-obese diabetic (NOD) mice, and as a protective negative regulator in autoimmune disorders.

1 mg/ml

Recombinant mouse IDO.

indoleamine 2, 3-dioxygenase 1

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.