IL-32 Antibody

Blue Ice

Predicted: 26kD
Observed: 26 kD

IL-32 Antibody: Interleukin-32 (IL-32) was initially identified as a transcript (NK4) that is selectively expressed in lymphocytes and NK cells and whose expression is increased following activation by IL-2. It was later re-isolated from an IL-18-treated lung carcinoma cell line and re-named IL-32. IL-32 is unusual in that it does not share sequence homology with known cytokine families and is highly expressed in immune tissues, existing in at least four differentially spliced isoforms. Because treatment of human monocytic and mouse macrophage cells with IL-32 induces several proinflammatory cytokines such as TNF-α , IL-8 and MIP-2, and because it is also induced in human peripheral lymphocyte cells after mitogen stimulation and in epithelial cells by IFNγ , it has been suggested that IL-32 may play a role in autoimmune and inflammatory diseases such as rheumatoid arthritis.

Trial Size

14424787

Human IL-32 has 7 isoforms, including isoform 1 (234aa, 27kD), isoform 2 (188aa, 22kD), isoform 3 (178aa, 21kD), isoform 4 (131aa, 15kD), isoform 5 (168aa, 19kD), isoform 6 (179aa, 21kD) and isoform 7 (148aa, 17kD). 3749 can detect all human isoforms.

PRS-1306-0.1mg

1 mg/mL

WB: 0.5-4 μ g/mL; IHC: 2-10 μ g/mL; IF: 20 μ g/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human and mouse samples; Immunofluorescence in human samples. All other applications and species not yet tested.

Anti-IL-32 antibody (3749) was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human IL-32.

The immunogen is located within the last 50 amino acids of IL-32.

interleukin 32

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.