GAPDH Antibody (HRP)

Blue Ice

Predicted: 37 kDa
Observed: 36 kDa

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. It also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export (1). GAPDH also plays a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer's and Huntington's disease (2). GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

None

HRP-GAPDH antibody is affinity chromatography purified via peptide column.

1 mg/mL

HRP-GAPDH antibody can be used for detection of GAPDH by Western blot at 0.5 - 1 μ /ml.
Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested.

HRP-GAPDH antibody was raised against a synthetic peptide containing 16 amino acids near the carboxy terminus of GAPDH.

glyceraldehyde-3-phosphate dehydrogenase

Optimal dilutions for each application to be determined by the researcher.