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IL-2 Antibody

ArtNr PRS-38-204-0.1mg
Hersteller ProSci
Menge 0.1 mg
Kategorie
Typ Antibody Primary
Format Lyophilized
Applikationen WB, ELISA, NT
Specific against Rat (Rattus norvegicus)
Host Rabbit
Konjugat/Tag Unconjugated
NCBI Il2
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias Il-2,Interleukin-2,T-cell growth factor,IL-2
Versandbedingung Raumtemperatur
Lieferbar
Manufacturer - Type
Primary Antibodies, Functional Antibodies, Antibody Pairs
Manufacturer - Applications
ELISA, WB, Neutralization Assay
Shipping Temperature
Room Temp
Storage Conditions
IL-2 antibody is stable for at least 2 years from date of receipt at -20˚ C. The reconstituted antibody is stable for at least two weeks at 2-8˚ C. Frozen aliquots are stable for at least 6 months when stored at -20˚ C. Avoid repeated freeze-thaw cycles.
Manufacturer - Research Area
Immunology, Chemokines & Cytokines, Antibody Pairs, Functional Assays
Background
Interleukin 2 (IL2) is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. IL2 has been shown to have antitumor effects in some studies. This is probably mediated by cytotoxic effector cells.
By Type
Immunology, Chemokines & Cytokines
Modifications
None
Protein Gi #
124328
Concentration
batch dependent
Application Note
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Rat IL-2 (1.0 ng/mL), a concentration of < 0.1 μ g/mL of this antibody is required.
ELISA:
Indirect:
To detect Rat IL-2 by indirect ELISA (using 100 μ L/well antibody solution) a concentration of 0.5 - 2.0 μ g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-2.
Sandwich
To detect Rat IL-2 by sandwich ELISA (using 100 μ L/well antibody solution) a concentration of 0.5 - 2.0 μ g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Rat IL-2 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-2.
Western Blot:
To detect Rat IL-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Immunogen
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat IL-2. Rat IL-2 specific antibody was purified by affinity chromatography employing immobilized Rat IL-2 matrix.
Clonality
Polyclonal
NCBI Official Name
interleukin 2
NCBI Organism
Rattus norvegicustus norvegicus
Disclaimer
This product is for research use only.
User Note
Centrifuge vial prior to opening.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 0.1 mg
Lieferbar: In stock
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