IFN-beta Antibody

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Infectious Disease, Neuroscience, Cancer, Antibody Pairs

None

IFN-beta specific antibody was purified by affinity chromatography employing immobilized Human IFN-beta matrix.

ELISA:
Sandwich:
To detect Human IFN-beta by sandwich ELISA (using 100 μ L/well antibody solution) a concentration of 0.5 - 2.0 μ g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Polyclonal Biotinylated Anti-Human IFN-beta (38-219) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IFN-beta.
Western Blot:
: To detect Human IFN-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μ g/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IFN-beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Human IFN-beta (5.00μ g/mL), a concentration of 2.5 μ g/mL of this antibody is required.

Polyclonal

Homo sapiens

Centrifuge vial prior to opening.