IL-33 Antibody

Blue Ice

Predicted: 31kD
Observed: 33kD

IL-33 Antibody: Interleukin-33 (IL-33) is a recently identified member of the IL-1 family of cytokines whose other members include IL-1α /beta, IL-1Ra and IL-18. Its receptor has been shown to be ST2, an IL-1 receptor family member that also acts as a negative regulator of TLR-IL-1R signaling and IL-1R accessory protein (IL-1RAcP). Receptor binding of IL-33 activates NF-κ B and MAP kinases and induces the expression of TH2-associated cytokines such as IL-4, IL-5 and IL-6. Prolonged IL-33 treatment of mice led to the development of eosinophilia, splenomegaly, and severe pathological changes in mucosal organs such as lungs, esophagus and small intestine. Recent experiments have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties.

Trial Size, Cell Treatment, Most Cited

15559209

Human IL-33 has 4 isoforms, including isoform 1 (270aa, 30.8kD), isoform 2 (228aa, 25.9kD), isoform 3 (228aa, 26.1kD) and isoform 4 (144aa, 16.3kD). This antibody detects only human isoform L. Mouse IL-33 has 1 isoform (266aa, 30kD). Rat IL-33 has only one isoform identified so far (264aa, 29.5kD).

PRS-1288-0.1mg

1 mg/mL

WB: 1-2 μ g/mL; IF: 20 μ g/mL; IHC-P: 2.5 μ g/mL.
Antibody validated: Western Blot in human and mouse samples; Immunofluorescence and Immunohistochemistry in human samples. All other applications and species not yet tested.

Anti-IL-33 antibody (4273) was raised against a 17 amino acid synthetic peptide from near the center of human IL-33.

The immunogen is located within the 150-200 amino acids of IL-33.

interleukin 33

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.