Vergleich

calbindin D28 Antibody

ArtNr PRS-43-170-0.1mg
Hersteller ProSci
Menge 0.1 mg
Kategorie
Typ Antibody Polyclonal
Format Liquid
Applikationen WB, IHC, ELISA
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host Caprine
Konjugat/Tag Unconjugated
Citations Kusakabe KT, Abe H, Kondo T, Kato K, Okada T, Otsuki Y.DNA microarray analysis in a mouse model for endometriosis and validation of candidate factors with human adenomyosis.
NCBI calbindin D28
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias CALB1,calbindin 1,28kDa,CALB,D-28K,OTTHUMP00000166027,OTTHUMP00000225441,RTVL-H protein,calbindin,calbindin 1,(28kD),calbindin D28,vitamin D-dependent calcium-binding protein,avian-type
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
Primary Antibodies
Shipping Temperature
Blue Ice
Storage Conditions
Aliquot and store at -20˚ C. Minimize freezing and thawing.
Molecular Weight
Approx 26kDa
Manufacturer - Research Area
Cancer, Immunology, Obesity,
By Type
Cancer, Immunology, Obesity,
Modifications
None
Protein Gi #
115394
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Buffer
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Application Note
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 26kDa band observed in Human Brain (Cerebellum) lysates and in Mouse and Rat total Brain lysates (calculated MW of 30.0kDa according to NP_004920.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Parmentier et al, Eur J Biochem. 1987 Dec30; 170(1-2):207-15. PMID: 3691519. Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Pig Brain and Kidney for this particular batch.Immunohistochemistry:Frozen sections of Human Hypothalamus shows staining of Calbinding D2 neurons. Recommended concentration: 0.1-0.3ug/ml. Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.
Homology
Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Manufacturer - Specificity
Immunizing peptide overlaps EF hand 2, but not any calcium binding site.
Immunogen
The immunogen for this antibody is: C-KTFVDQYGQRDDGK
Clonality
Polyclonal
NCBI Official Name
calbindin 1, 28kDa
NCBI Organism
Homo sapiens
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 0.1 mg
Lieferbar: In stock
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