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calbindin D28 Antibody

calbindin D28 Antibody

Item no.	PRS-43-170-0.1mg
Manufacturer	ProSci
Amount	0.1 mg
Category	Oncology Neuroscience Neural Lineage Markers Molecular Targets for Neuroscience Antibodies Immunohistochemistry Western Blot ELISA Primary Antibodies By Organ Brain Cancer Calcium Binding Protein Markers Neurodegeneration Markers Microglia Markers
Type	Antibody Polyclonal
Format	Liquid
Applications	WB, IHC, ELISA
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Caprine
Conjugate/Tag	Unconjugated
Citations	Kusakabe KT, Abe H, Kondo T, Kato K, Okada T, Otsuki Y.DNA microarray analysis in a mouse model for endometriosis and validation of candidate factors with human adenomyosis.
NCBI	calbindin D28
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	CALB1,calbindin 1,28kDa,CALB,D-28K,OTTHUMP00000166027,OTTHUMP00000225441,RTVL-H protein,calbindin,calbindin 1,(28kD),calbindin D28,vitamin D-dependent calcium-binding protein,avian-type
Shipping Condition	Cool pack
Available
Manufacturer - Type	Primary Antibodies
Shipping Temperature	Blue Ice
Storage Conditions	Aliquot and store at -20˚ C. Minimize freezing and thawing.
Molecular Weight	Approx 26kDa
Manufacturer - Research Area	Cancer, Immunology, Obesity,
By Type	Cancer, Immunology, Obesity,
Modifications	None
Protein Gi #	115394
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration	500 ug/mL
Buffer	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Application Note	Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 26kDa band observed in Human Brain (Cerebellum) lysates and in Mouse and Rat total Brain lysates (calculated MW of 30.0kDa according to NP_004920.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Parmentier et al, Eur J Biochem. 1987 Dec30; 170(1-2):207-15. PMID: 3691519. Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Pig Brain and Kidney for this particular batch.Immunohistochemistry:Frozen sections of Human Hypothalamus shows staining of Calbinding D2 neurons. Recommended concentration: 0.1-0.3ug/ml. Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.
Homology	Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Manufacturer - Specificity	Immunizing peptide overlaps EF hand 2, but not any calcium binding site.
Immunogen	The immunogen for this antibody is: C-KTFVDQYGQRDDGK
Clonality	Polyclonal
NCBI Official Name	calbindin 1, 28kDa
NCBI Organism	Homo sapiens
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.1 mg

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Delivery expected until 2/5/2026

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