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Histamine Receptor H1 Antibody

Histamine Receptor H1 Antibody

Hersteller	ProSci
Kategorie	Oncology Food Safety Histamines Antibodies Immunohistochemistry ELISA Primary Antibodies By Organ Colorectal Cancer
Typ	Antibody Primary
Specific against	Human, Rat
Format	Liquid
Applikationen	IF, IHC, ELISA
Menge	0.1 mg
Host	Goat
ArtNr	PRS-45-731-0.1mg
eClass 6.1	32160702
eClass 9.0	32160702
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Shipping	blue ice or RT
By Research Area	Cancer
Homology	Expected Species Reactivity based on sequence homology: Mouse, Dog, Cow
Immunogen	The immunogen for this antibody is: CNENFKKTFKRILH
Applications	Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave bands at approx 55kDa and 37kDa in Human Duodenum lysate after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the 37kDa band we observe given the calculated size of 55.8kDa according to NP_000852.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional band).Immunohistochemistry:In paraffin embedded Human Colon shows membranous staining. Recommended concentration: 4-6ug/ml. Paraffin embedded Human Small Intestine. Recommended concentration: 5ug/ml. This product has been successfully used in IHC on Rat (PMID: 30226827).Immunofluorescence: This product has been successfully used in IF on Rat (PMID: 30413645).
Specificity	Variants (NP_000852.1; NP_001091681.1; NP_001091682.1; NP_001091683.1) encode the same protein.
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality	Polyclonal
Conjugate	Unconjugated
Buffer	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration	500 ug/mL
Storage Conditions	Aliquot and store at -20˚ C. Minimize freezing and thawing.
Related Products	Cat. No. 45-731P - Histamine Receptor H1 Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	HRH1
Accession #	NP_000852.1, NP_001091681.1, NP_001091682.1, NP_001091683.1
Protein Gi #	4504491
Ncbi Gene Id #	3269
Ncbi Official Symbol	HRH1
Ncbi Official Full Name	histamine receptor H1
Ncbi Organism	Homo sapiens
Swissprot #	P35367
Background References 1	Kawakami N, Miyoshi K, Horio S, Fukui H. Beta(2)-adrenergic receptor-mediated histamine H(1) receptor down-regulation: another possible advantage of beta(2) agonists in asthmatic therapy. J Pharmacol Sci. 2004 Apr; 94(4):449-58.
1st Image Caption	45-731 (4ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
2nd Image Caption	45-731 (5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

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Menge: 0.1 mg

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