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Histamine Receptor H1 Antibody

Histamine Receptor H1 Antibody

Item no.	PRS-45-731-0.1mg
Manufacturer	ProSci
Amount	0.1 mg
Category	Oncology Food Safety Histamines Antibodies Immunohistochemistry ELISA Primary Antibodies By Organ Colorectal Cancer
Type	Antibody Primary
Format	Liquid
Applications	IF, IHC, ELISA
Specific against	Human (Homo sapiens), Rat (Rattus norvegicus)
Host	Caprine
Conjugate/Tag	Unconjugated
Citations	Kawakami N, Miyoshi K, Horio S, Fukui H. Beta(2)-adrenergic receptor-mediated histamine H(1) receptor down-regulation: another possible advantage of beta(2) agonists in asthmatic therapy. J Pharmacol Sci. 2004 Apr;94(4):449-58.
NCBI	HRH1
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	HRH1, histamine receptor H1, HGNC:5182, H1-R, hisH1, histamine receptor, subclass H1, OTTHUMP00000160133, histamine H(1) receptor
Available
Manufacturer - Applications	Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave bands at approx 55kDa and 37kDa in Human Duodenum lysate after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the 37kDa band we observe given the calculated size of 55.8kDa according to NP_000852.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional band).Immunohistochemistry:In paraffin embedded Human Colon shows membranous staining. Recommended concentration: 4-6ug/ml. Paraffin embedded Human Small Intestine. Recommended concentration: 5ug/ml. This product has been successfully used in IHC on Rat (PMID: 30226827).Immunofluorescence: This product has been successfully used in IF on Rat (PMID: 30413645).
Shipping Temperature	blue ice or room temperature
Storage Conditions	Aliquot and store at -20°C. Minimize freezing and thawing.
Manufacturer - Research Area	Cancer
Protein Gi #	4504491
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration	500 ug/mL
Buffer	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Homology	Expected Species Reactivity based on sequence homology: Mouse, Dog, Cow
Immunogen	The immunogen for this antibody is: CNENFKKTFKRILH
Clonality	Polyclonal
NCBI Official Name	histamine receptor H1
NCBI Organism	Homo sapiens
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.1 mg

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