Hersteller |
ProSci
|
Kategorie |
|
Typ |
Antibody Primary |
Specific against |
Human |
Format |
Liquid |
Applikationen |
IHC, ELISA |
Menge |
0.1 mg |
Host |
Goat |
ArtNr |
PRS-45-948-0.1mg |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Cancer |
Homology |
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog |
Immunogen |
The immunogen for this antibody is: C-DKLEYLDEKQRDPR |
Applications |
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 80-90kDa double band in Human Placenta lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 67.7kDa according to NP_001040625.1. The 80-90kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Breast shows cytoplasm staining of myoepithelial cells of ducts. Recommended concentration, 2.5ug/ml. |
Specificity |
This antibody is expected to recognize both reported isoforms (NP_001040625.1 and NP_005854.2). |
Positive Control 1 |
Cat. No. 1311 - Human Breast Tissue Lysate |
Purification |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Clonality |
Polyclonal |
Conjugate |
Unconjugated |
Buffer |
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing. |
Concentration |
500 ug/mL |
Storage Conditions |
Aliquot and store at -20˚ C. Minimize freezing and thawing. |
Related Products |
Cat. No. 45-948P - NET1 Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
NET1 |
Accession # |
NP_005854.2, NP_001040625.1 |
Protein Gi # |
19923327 |
Ncbi Gene Id # |
10276 |
Ncbi Official Symbol |
NET1 |
Ncbi Official Full Name |
neuroepithelial cell transforming 1 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q7Z628 |
Background References 1 |
Qin H, Carr HS, Wu X, Muallem D, Tran NH, Frost JA. Characterization of the biochemical and transforming properties of the neuroepithelial transforming protein 1. J Biol Chem. 2005 Mar 4; 280(9):7603-13. Epub 2004 Dec 16. |
1st Image Caption |
45-948 (2.5ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.