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Comparison

NET1 Antibody

Item no. PRS-45-948-0.1mg
Manufacturer ProSci
Amount 0.1 mg
Category
Type Antibody Primary
Format Liquid
Applications IHC, ELISA
Specific against Human (Homo sapiens)
Host Caprine
Conjugate/Tag Unconjugated
Citations Qin H, Carr HS, Wu X, Muallem D, Tran NH, Frost JA. Characterization of the biochemical and transforming properties of the neuroepithelial transforming protein 1. J Biol Chem. 2005 Mar 4;280(9):7603-13. Epub 2004 Dec 16.
NCBI NET1
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias NET1, ARHGEF8, neuroepithelial cell transforming gene 1, NET1A, Rho guanine nucleotide exchange factor (GEF) 8, guanine nucleotide regulatory protein (oncogene), neuroepithelioma transforming gene 1, p65 Net1 proto-oncogene protein, small GTP-binding prot
Shipping Condition Cool pack
Available
Manufacturer - Type
Primary Antibodies
Shipping Temperature
Blue Ice
Storage Conditions
Aliquot and store at -20˚ C. Minimize freezing and thawing.
Manufacturer - Research Area
Cancer
By Type
Cancer
Modifications
None
Protein Gi #
19923327
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Positive Control 1
PRS-1311-0.1mg
Concentration
500 ug/mL
Buffer
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Application Note
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 80-90kDa double band in Human Placenta lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 67.7kDa according to NP_001040625.1. The 80-90kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Breast shows cytoplasm staining of myoepithelial cells of ducts. Recommended concentration, 2.5ug/ml.
Homology
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Manufacturer - Specificity
This antibody is expected to recognize both reported isoforms (NP_001040625.1 and NP_005854.2).
Immunogen
The immunogen for this antibody is: C-DKLEYLDEKQRDPR
Clonality
Polyclonal
NCBI Official Name
neuroepithelial cell transforming 1
NCBI Organism
Homo sapiens
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Manufacturer - Additional Names
NET1, ARHGEF8, neuroepithelial cell transforming gene 1, NET1A, Rho guanine nucleotide exchange factor (GEF) 8, guanine nucleotide regulatory protein (oncogene), neuroepithelioma transforming gene 1, p65 Net1 proto-oncogene protein, small GTP-binding protein regulator

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.1 mg
Available: In stock
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