ATG5 Antibody

Blue Ice

Predicted: 32kD
Observed: 55kD (ATG5-ATG12 conjugation)

ATG5 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG5, another member of the autophagy protein family, forms a conjugate with ATG12; this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells.

Trial Size, IAV

119568800

Human ATG5 has 2 isoforms, including isoform L (275aa, 32.4kD) and isoform S (197aa, 22.9kD. This antibody detects only human isoform L. Mouse ATG5 has 1 isoform (275aa, 32.4kD). Rat ATG5 has only one isoform identified so far (275aa, 32.4kD).

PRS-1406-0.1mg

1 mg/mL

WB: 1-2 μ g/mL; IHC-P: 2.5 μ g/mL.
Antibody validated: Western Blot in human and mouse samples, Immunohistochemistry in human samples. All other applications and species not yet tested.

Three isoforms of ATG5 are known to exist; this ATG5 antibody will only detect the longest isoform.

Polyclonal

Homo sapiens

Optimal dilutions for each application to be determined by the researcher.