Hersteller |
ProSci
|
Kategorie |
|
Typ |
Antibody Polyclonal |
Specific against |
Human |
Format |
Liquid |
Applikationen |
WB, IF, IHC-P |
Menge |
400 ul |
Host |
Rabbit |
ArtNr |
PRS-55-787-400ul |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Signal Transduction |
Homology |
Predicted species reactivity based on immunogen sequence: Chicken, Hamster, Mouse |
Immunogen |
This COPE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 273-300 amino acids from the C-terminal region of human COPE. |
Applications |
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50 |
Predicted Molecular Weight |
34 kDa |
Purification |
This antibody is purified through a protein A column, followed by peptide affinity purification. |
Clonality |
Polyclonal |
Isotype |
Rabbit Ig |
Conjugate |
Unconjugated |
Buffer |
Supplied in PBS with 0.09% (W/V) sodium azide. |
Concentration |
batch dependent |
Storage Conditions |
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
COPE |
Accession # |
O14579 |
Ncbi Gene Id # |
11316 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
COPE |
Ncbi Official Full Name |
Coatomer subunit epsilon |
Ncbi Organism |
Homo sapiens |
Swissprot # |
O14579 |
Background |
The product of this gene is an epsilon subunit of coatomerprotein complex. Coatomer is a cytosolic protein complex that bindsto dilysine motifs and reversibly associates with Golginon-clathrin-coated vesicles. It is required for budding from Golgimembranes, and is essential for the retrograde Golgi-to-ERtransport of dilysine-tagged proteins. Coatomer complex consists ofat least the alpha, beta, beta', gamma, delta, epsilon and zetasubunits. Alternatively spliced transcript variants encodingdifferent isoforms have been identified. |
Background References 1 |
Davila, S., et al. Genes Immun. 11(3):232-238(2010) |
Background References 2 |
Morikawa, R.K., et al. J. Biol. Chem. 284(39):26620-26630(2009) |
Background References 3 |
Maruyama, S., et al. Mol. Cell. Biochem. 307 (1-2), 73-82 (2008) : |
Background References 4 |
Goryachev, A.B., et al. PLoS Comput. Biol.(12), E172 (2006) : |
1st Image Caption |
Western Blot at 1:1000 dilution Lane 1: A375 whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane. |
2nd Image Caption |
COPE Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. |
3rd Image Caption |
Confocal immunofluorescent analysis of COPE Antibody with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.