ATG3 Antibody

Primary Antibodies

ATG3 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

ATG3 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG3 (APG3) is a widely expressed conjugating enzyme for APG8 lipidation, an essential step for the initiation of autophagy. It functions as an E2-like enzyme during the initial stages of autophagosome formation by catalyzing the formation of the Atg8-phosphatidylethanolamine (Atg8-PE) conjugate, which is critical for autophagy.

Trial Size

19526773

PRS-1405-0.1mg

1 mg/mL

ATG3 antibody can be used for detection of ATG3 by Western blot at 1 - 2 μ g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ g/mL. For immunofluorescence start at 20 μ g/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

ATG3 antibody was raised against an 18 amino acid synthetic peptide near the center of human ATG3.

The immunogen is located within amino acids 180 - 230 of ATG3.

ATG3 autophagy related 3 homolog (S. cerevisiae)

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.