Hersteller |
ProSci
|
Kategorie |
|
Typ |
Antibody Polyclonal |
Specific against |
Human |
Format |
Liquid |
Applikationen |
WB, IHC-P |
Menge |
400 ul |
Host |
Rabbit |
ArtNr |
PRS-63-101-400ul |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Lieferbar |
|
Shipping |
blue ice or RT |
By Research Area |
Signal Transduction |
Homology |
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat |
Immunogen |
This ILK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 225-253 amino acids from human ILK. |
Applications |
For IHC-P starting dilution is: 1:50~100
For WB starting dilution is: 1:1000 |
Predicted Molecular Weight |
51 kDa |
Purification |
This antibody is purified through a protein A column, followed by peptide affinity purification. |
Clonality |
Polyclonal |
Isotype |
Rabbit Ig |
Conjugate |
Unconjugated |
Buffer |
Supplied in PBS with 0.09% (W/V) sodium azide. |
Concentration |
batch dependent |
Storage Conditions |
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
ILK |
Accession # |
Q13418 |
Protein Gi # |
9973397 |
Ncbi Gene Id # |
3611 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
ILK |
Ncbi Official Full Name |
Integrin-linked protein kinase |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q13418 |
Background |
Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. ILK is a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. |
Background References 1 |
Li, Y., et al., J. Clin. Invest. 112(4):503-516 (2003). |
Background References 2 |
Troussard, A.A., et al., J. Biol. Chem. 278(25):22374-22378 (2003). |
Background References 3 |
Marotta, A., et al., Br. J. Cancer 88(11):1755-1762 (2003). |
Background References 4 |
Cordes, N., et al., Br. J. Cancer 88(9):1470-1479 (2003). |
1st Image Caption |
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ILK Antibody (S246) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. |
2nd Image Caption |
Western blot analysis of anti-ILK Antibody (S246) in Hela cell line lysates (35ug/lane) |
3rd Image Caption |
Western blot analysis of ILK using rabbit polyclonal ILK Antibody (pS246) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ILK gene. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.