ULK3 Antibody

Blue Ice

Predicted: 52 kDa
Observed: 52 kDa

ULK3 belongs to the Ser/Thr protein kinase superfamily and plays a role in the ATP-dependent phosphorylation of target proteins (1). Knockout of ULK genes results in a severe defect in the autophagy pathway (2). ULK3, like the other Unc-51-like kinases such as ULK1, ULK2 and ULK4, is highly conserved among eukaryotes (3). ULK3 has been shown to be a positive regulator of the Hedgehog signaling pathway by enhancing GLI1 and GLI2 transcriptional activity (4). Furthermore, ULK3 can also interact with SUFU, a protein required for the negative regulation of GLI proteins; this interaction blocks the autophosphorylation of ULK3 and blocks its ability to regulate the GLI proteins (5).

Trial Size

150456432

PRS-1303-0.1mg

1 mg/mL

ULK3 antibody can be used for detection of ULK3 by Western blot at 0.5 - 1 μ g/ml. Antibody can also be used for Immunohistochemistry starting at 5 μ g/mL. For immunofluorescence start at 20 μ g/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

ULK3 antibody is human specific. Multiple isoforms of ULK3 are known to exist.

Polyclonal

homo sapiens

Optimal dilutions for each application to be determined by the researcher.